比普通DIG隨機引物法標記效率高,采用CSPO化學發(fā)光檢測靈敏度更高.一個kit可用于12次標記反應和24次檢測反應組成;5×Dig標記復合物;未標記對照DNA,PBR328;DNA稀釋液;抗-Dig-堿性磷酸酶連接物;CSPD;10×封閉液;Dig定量試紙條;Dig對照試紙條;NBT/BCIP儲存液,-20℃保存
Catalog # Product name / Packsize 
11585614910

（1585614）  DIG-High Prime DNA Labeling and Detection Starter Kit II

1 kit （12 labeling reactions and 24 detection reactions） 
 
Application
DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits
The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.

Product Description
Sensitivity and specificity: Standard assay uses 1 μg of linearized pBR328. It is usual to expect 0.8 μg labeled DNA after 1 h of labeling, and 2.3 μg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence （probe concentrations at 20 ng/ml）. A single copy human gene （t-PA） is detectable in a Southern blot, loading 1 μg of digested placenta DNA.

Contents
 DIG-High Prime, 5x conc., 50 μl
 DIG-labeled Control DNA, 20 μl, （5 μg/ml） pBR328 （linearized with Bam HI）
 DNA Dilution Buffer, 3 x 1 ml
 Anti-digoxigenin-AP Conjugate, 50 μl
 CSPD, ready-to-use, 50 ml
 Blocking Solution, 10x conc., 4 x 100 ml
 DIG Easy Hyb Granules, 4 x 100 ml 
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